@article{ef532de0dccb462983c9568e4993b5bc,
title = "Variations in DNA methylation patterns during the cell cycle of HeLa cells",
keywords = "Cancer, Cell cycle, CpG microarray, DNA methylation, Epigenetics, Repetitive sequences",
author = "Brown, {Shelley E.} and Fraga, {Mario F.} and Weaver, {Ian C.G.} and Maria Berdasco and Moshe Szyf",
note = "Funding Information: withtheFACSscanningandsortingandTracey It has long been known that DNA methylation patterns undergo dramatic changes RigbyattheBiotechnologyResearchInstitute during development but it was assumed that once tissue specific methylation patterns for her help with the microarray hybridiza- are formed and cell fate is determined, the pattern of methylation is accurately copied tionandscanning.S.E.B.issupportedbya during DNA synthesis by the semi-conservative DNMT1.2 DNMT1 was shown to prefer fellowship from the CIHR.This study was a hemi-methylated substrate, methylated on one strand, to a substrate unmethylated on funded by a grant awarded to M.S. by the both strands.10Thus, DNMT1 would only methylate a CG on the nascent strand of NCIC. DNA if the opposite CG on the parental strand was methylated, resulting in accurate copying of the DNA methylation pattern of the parental strand onto the nascent strand. DNMT1 is associated with the DNA replication fork11 and DNA methylation occurs concurrently with DNA replication.12This process prevents either introduction of new methylation sites or loss of methylated residues in mature dividing cells. In addition, the {\textcopyright}2007 LANDES BIOSCIENCEDNAmethylationpatterninnondividingcellsisbelievedtobeconservedsinceactive removal of methyl groups from DNA does not exist in somatic cells. Together, the combi-nation of a semi-conservative DNMT in dividing cells and the lack of demethylase activity in nondividing cells were previously believed to ensure that the DNA methylation pattern is faithfully maintained throughout life.",
year = "2007",
doi = "10.4161/epi.2.1.3880",
language = "English",
volume = "2",
pages = "54--65",
journal = "Epigenetics",
issn = "1559-2294",
publisher = "Landes Bioscience",
number = "1",
}
