Altering the Y137-K164-K166 triad of mandelate racemase and its effect on the observed pK_a of the Brønsted base catalysts

Biochemistry, Genetics and Molecular Biology

• Amino Acids 100%
• Active Site 100%
• Site-Directed Mutagenesis 100%
• Wild Type 100%
• Enantiomer 100%
• Static Electricity 100%
• Turnover Number 100%
• Catalytic Efficiency 100%

Chemistry

• Base Catalyst 100%
• Mandelate 100%
• (R)-Mandelate 33%
• Kcat 16%
• pKa 16%
• (S)-Mandelate 16%
• Kcat/Km 16%

Keyphrases

• Base Catalyst 100%
• H-bond Network 40%
• Rate Study 20%
• Interconversion 20%
• NH3+ Groups 20%
• Catalytic Residues 20%
• Conjugate Acids 20%
• PH-rate Profile 20%