Current strategies for protein production and purification enabling membrane protein structural biology¹

Keyphrases

• Protein Purification 100%
• Membrane Protein 100%
• Protein Production 100%
• Specimen Preparation 20%
• Low Abundance 20%
• Overexpressing 20%
• Heterologous Expression 20%
• Structural Characterization 20%
• Protein Expression 20%
• X-ray Crystallography 20%
• NMR Spectroscopy 20%
• Exponential Growth 20%
• Purification Process 20%
• Protein Sample 20%
• Membrane Protein Structure 20%
• Protein Isolation 20%
• Natural Host 20%
• Cell-free Expression 20%
• Production Methods 20%
• Novel Protein 20%
• Single Bottleneck 20%
• Multiple Obstacles 20%
• Cryogenic Electron Microscopy 20%
• In Vitro Translation 20%
• Membrane Protein Structural Biology 20%
• Protein Data Bank 20%
• Heterologous System 20%
• Superhydrophobic 20%

Biochemistry, Genetics and Molecular Biology

• Copurification 100%
• Transmembrane Protein 100%
• Membrane Protein 100%
• Magnetism 25%
• Heterologous Expression 25%
• Protein Isolation 25%
• Natural Host 25%
• X-Ray Crystallography 25%
• Cryo-Electron Microscopy 25%
• Growth 25%
• RNA Translation 25%
• Protein Data Bank 25%

Neuroscience

• Structural Protein 100%
• Transmembrane Protein 100%
• In Vitro 25%
• Heterologous Expression 25%
• Nuclear Magnetic Resonance Spectroscopy 25%
• Amino Acid Activation 25%
• Cryo-Electron Microscopy 25%
• Protein Structure 25%